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The Effect of Infusion Tubing Material and Flow Rate on the Viscosity and Turbidity of Human Serum Albumin
Marlyn Dian Laksitorini, Sinta Wahyu Septiani, Abdul Rohman
= https://doi.org/10.26753/jfks.v5i1.1645
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Abstract
Human Serum Albumin (HSA) is a therapeutic protein widely administered intravenously in clinical practice. Due to its inherently sensitive structure, HSA is susceptible to physical instabilities such as denaturation and aggregation, which can compromise its therapeutic efficacy. Factors associated with the infusion process, including the tubing material and flow rate, are suspected to contribute to this degradation.
Objective
This study aims to evaluate the effects of infusion tubing materials and flow rates on the physical stability of HSA, assessed through changes in viscosity and turbidity.
Methods
An experimental study was conducted using commercially available HSA 20% preparations passed through two types of infusion tubing materials—polyvinyl chloride (PVC) and acrylic butadiene styrene (ABS)—at flow rates of 2 mL/min and 3 mL/min. Viscosity was measured using an Ostwald viscometer, while turbidity was assessed at 350 nm and 600 nm wavelengths via UV-Vis spectrophotometry. Untreated samples served as controls, and each experimental condition was performed in triplicate.
Results
The interaction between HAS and infusion tubing material significantly affects HSA's viscosity and turbidity. ABS and PVC have a similar degree of magnitude in reducing HSA's viscosity and increasing the turbidity. Increased infusion rate had a greater effect on viscosity than on turbidity. The turbidity examination at 600 nm is more sensitive than at 350 nm.
Conclusion
Interaction with the tubing material increased the HAS's turbidity and reduced its viscosity. Under the current experimental condition of interacting commercial HSA with PVC or ABS tubing material under the flow rates of 2 ml/min and 3 ml/min, the type of polymer material has no significant effect on HSA’s viscosity and turbidity. Higher flow rates provide more changes in viscosity and, to a lesser extent, turbidity. The combined measurement of viscosity and turbidity offers a practical and non-destructive screening method for evaluating protein stability.
Keywords
References
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